Figure 6.

LINC00473 regulates IL-1β-stimulated C28/I2 apoptosis and the inflammatory response by regulating LY6E. (A) Overexpression efficacy of LY6E in C28/I2 cells was determined using reverse transcription-quantitative PCR. (B and C) Flow cytometry was performed to assess apoptosis in transfected C28/I2 cells. Protein expression levels of caspase-3, Bax and Bcl-2 in C28/I2 cells under the indicated transfections were (D) detected by western blotting and (E) semi-quantified. Concentrations of inflammatory cytokines (F) IL-6, (G) IL-8 and (H) TNF-α were measured using ELISAs in transfected C28/I2 cells. ^*P<0.05. LINC00473, long intergenic non-protein coding RNA 473; LY6E, lymphocyte antigen 6 locus E; si, small interfering RNA; NC, negative control; PI, propidium iodide