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. 2021 Sep 2;22(5):1244. doi: 10.3892/etm.2021.10679

Figure 6.

Figure 6

Decreased TLR4 is identified as a suppressor of OA inflammatory factors in vitro. (A) The mRNA and (B) protein expression level of TLR4 following transfection with sh-TLR4 or pcDNA-TLR4 and their respective negative controls into the HC-a was detected using reverse transcription-quantitative PCR and western blot analysis respectively. ***P<0.001 vs. the sh-NC group. ###P<0.001 vs. the pcDNA3.1 group. The concentration of (C) IL-6, (D) TNF-α and (E) PGE2 in IL-1β-induced HC-a was measured using ELISA. ***P<0.001 vs. the Control group. ###P<0.01 vs. the IL-1β + sh-NC group. &&P<0.01 vs. the IL-1β + pcDNA3.1 group. &&&P<0.001 vs. the IL-1β + pcDNA3.1 group. NC, negative control; miR, microRNA; TNF, tumor necrosis factor; PGE2, prostaglandin E2; TLR4, toll-like receptor 4.