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. 2021 Sep 2;22(5):1244. doi: 10.3892/etm.2021.10679

Figure 7.

Figure 7

Knockdown of LINC01385 suppresses the development of OA by regulating the miR-140-3p/TLR4 axis in vitro. (A) The protein expression level of TLR4 following transfection with sh-LINC01385, sh-LINC01385 + miR-140-3p inhibitor or sh-LINC01385 + pcDNA-TLR4 into the HC-a was measured using western blot analysis. ***P<0.001 vs. the sh-NC group. ##P<0.01, ###P<0.001 vs. the sh-LINC01385 group. (B) The viability of the IL-1β-induced HC-a was measured using a MTT assay. ***P<0.001 vs. the sh-NC group. ##P<0.01 vs. the sh-LINC01385 group. The concentration of (C) IL-6, (D) TNF-α and (E) PGE2 in IL-1β-induced HC-a was measured using ELISA. ***P<0.001 vs. the sh-NC group. ##P<0.01, ###P<0.001 vs. the sh-LINC01385 group. NC, negative control; miR, microRNA; sh, short hairpin; TNF, tumor necrosis factor; PGE2, prostaglandin E2; TLR4, toll-like receptor 4.