Figure 3.

Cross-reactivity analyses indicate antigen-dependent TCR docking on HLA peptide complexes. (A–C) TCR cross-reactivity was tested by quantification of secreted IFN-γ on coculturing TCR-transduced T cells with T2 target cells pulsed with ala/thr scanned peptide cognates (1 µM peptide) of ligands SYTL4^S363F (A), KIF2C^P13L (B), and NCAPG2^P333L (C). IFN-γ secretion values from single conditions were normalized against the cytokine level in response to the defined neoantigen. HLA, human leukocyte antigen; IFN, interferon; TCR, T-cell receptor.