Figure 3.

SINV-CAR targeted with tFab generates functional CAR-T cells directly in vivo. (A) Experimental schema of the mouse model. Following a lose dose of irradiation (100 rad), mice were injected with FFLuc BV-173 (5×10⁵ cells) intravenously. Five days later, mice were injected intravenously with 5×10⁶ activated PBMCs followed by either SINV-CAR alone or SINV-CAR plus tFab 30 min later. (B) Representative tumor bioluminescence (BLI) (color scale: min=1×10⁶; max=5×10⁷) for mice treated according to scheme from panel A. (C) BLI kinetics for all mice treated according to scheme from panel A. Lighter lines represent individual mice, while bolded lines represent the means for the treatment groups. Summary of two independent experiments (n=10 mice for each condition). ***P=0.0002; ****p<0.0001, two-way ANOVA with Bonferroni correction. (D) Kaplan-Meier survival curve for all mice (n=10 mice per condition) treated according to scheme from panel A. *P=0.0242, log-rank test. (E) Representative flow plots (left panel) and quantification (right panel) of CAR-T cells in the CD3⁻ (open symbols) and CD3⁺ (filled symbols) populations (gated on live cells) in the peripheral blood at the time of euthanasia (n=10 each condition, mean shown). *P=0.0100 and *p=0.0202, unpaired t-test. ANOVA, analysis of variance; CAR, chimeric antigen receptor; PBMCs, peripheral blood mononuclear cells.