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. 2021 Sep 8;11(4):20458940211041512. doi: 10.1177/20458940211041512

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 4. — Effect of right heart catheterization on angiography parameters in the peripheral regions of the mouse lungs. (a and b) Representative angiograph images of the whole left lung at 8× magnification and the peripheral regions of the apical (Api, 1), middle (Mid, 2) and basal (Bas, 3) areas of the left lung at 30× magnification (left panels) from mice without (-, a) or with (+, b) right heart catheterization (RH Cath or RHC). Enlarged angiograph images of the labeled Api, Mid and Bas areas (30×) depicting the most peripheral region (A) and the adjacent region (B) from mice without (–RHC, a) or with (+RHC, b) right heart cath. (c and e) Summarized data (mean±SE, n=16) showing the total length of lung vascular branches (upper panels), the number of lung vascular branches (middle panels) and the number of lung vascular branch junctions (lower panels) per square millimeter from the selected Api, Mid and Bas lung areas (c), the selected most peripheral areas (A) and adjacent peripheral areas (B) in the Api, Mid and Bas lung regions (d), and the combined (A+B) peripheral areas in the Api, Mid and Bas lung regions (e) from –RHC mice (blue) and +RHC mice (red). (f) Representative angiograph images showing the whole left lung at 8× and 12.5× magnification from –RHC and +RHC mice. (g) Summarized data (mean±SE, n=16) showing the total length of lung vascular branches (left panel), the number of lung vascular branches (center panel) and the number of lung vascular branch junctions (right panel) per square millimeter of the whole-lung images from –RHC mice (blue) and +RHC mice (red). The –RHC and +RHC mice include both male and female mice.