Table 1.

Information of plastome assembly, annotation, number of genomic rearrangements, and percent repetitive elements

Genus	Species	No. of individuals	Entire	Overall		No. of	No. of rRNA genes	No. of tRNA genes	No. of genomic rearrangements	Percent repetitive DNA (%)b
			plastome	GC	No. of	Protein-
			size	content	genes	coding
			(bp)	(%)		genes
Medicago	M. polymorpha	5	124,247-124,445	34.0, 34.1	110	75	4	30	5	4.41–4.56
	M. truncatula	2	123,391-123,767	34.0	110	76	4	30	4, 5	4.00–4.16
	M. sativa	3	125,523-125,623	33.8, 33.9	110	76	4	30	4	4.13–4.55
	M. lupulinaa	3	122,194-122,310	34.1	110	76	4	30	9	5.93–6.02
	M. minimaa	5	132,071-132,219	34.2	110	75, 76	4	30	7, 8	4.99–5.06
	M. ruthenica	5	127,065-127,674	34.2	112	76	4	32	8	5.53–6.07
	M. archiducis-nicolai	4	126,635-126,810	34.1	112	76	4	32	7	5.20–5.38
	M. platycarpos	2	125,502-125,528	34.1	110	76	4	30	6	4.27
	M. falcata	3	125,357-125,555	33.8, 33.9	110	76	4	30	5	4.17–4.22
	M. edgeworthii	5	122,454-122,549	33.9, 34.0	110	76	4	30	4	4.01–4.09
	M. monantha	3	121,336-121,358	34.1	110	76	4	30	5	3.43–3.46
	M. orthoceras	5	121,043-121,065	34.1	110	76	4	30	5	2.98–3.11
	M. arcuata	2	121,728-121,777	34.0	110	76	4	30	5	3.54–3.57
	M. cancellata	5	121,889-121,953	34.0	110	76	4	30	5	3.57–3.63
Trigonella	T. cachemiriana	3	125,555	34.0	110	76	4	30	6	3.96–4.04
	T. emodi	5	128,493-128,643	33.8	110	76	4	30	9	6.19–6.29
Melilotus	Melilotus dentataa	4	141,922-142,713	33.7, 33.8	111	76	4	30	6	4.62–4.94
	Melilotus indicus	3	127,703-128,044	33.6	112	76	4	31	7	6.07–6.39
	Melilotus officinalis	4	126,534-127,451	33.7	111,112	76	4	31	5, 6	5.43–6.13
	Melilotus alba	4	127,293-127,694	33.6, 33.7	111, 112	76	4	31	6, 7	5.96–6.25
Total	20	75	121,043-132,219	33.6–34.1	110–112	75–76	4	30–32	4–9	2.98–6.39

^a GC content, No. of genes, protein-coding genes, rRNA genes, tRNA genes, and Percent repetitive DNA were calculated using only one IR copy

^bTandem repeats and dispersed repeats ≥30 bp