Fig. 2.

miR-146a-5p inhibits biological properties and EMT of trophoblast cells. Note: After HTR-8 cells were transfected with miR-146a-5p mimic, miR-146a-5p inhibitor, mimic NC or inhibitor NC, RT-qPCR was used to detect miR-146a-5p expression (A); MTT assay (B) and colony formation assay (C) determined the proliferative capacity of HTR-8 cells; invasiveness of HTR-8 cells as measured by Transwell invasion assay (D); HTR-8 cell migration was evaluated by Scratch assay (E); RT-qPCR and Western blotting examined the expression levels of Vimentin, N-cadherin and E-cadherin (F, G). All data are presented as mean  ±  SDs; n  =  3, *P  <  0.05, **P  <  0.01, ***P  <  0.001. SD standard deviation; EMT epithelial-mesenchymal transition