Fig. 5.

miR-146a-5p regulates Wnt2 to affect trophoblast cell progression and EMT process. Note: After HTR-8 cells were transfected or cotransfected with miR-146a-5p mimic or pcDNA3.1-Wnt2, the mRNA and protein levels of Wnt2 were measured by RT-qPCR (A) and Western blotting (B); MTT assay and colony formation assay detected the proliferation of HTR-8 cells (C, D); invasiveness of HTR-8 cells as measured by Transwell invasion assay (E); HTR-8 cell migration was assessed by Scratch assay (F); RT-qPCR and Western blot analyses for EMT-related proteins (G, H). All data are presented as mean  ±  SDs; n  =  3, *P  <  0.05, **P  <  0.01. SD standard deviation; EMT epithelial-mesenchymal transition