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. 2021 Sep 14;19:93. doi: 10.1186/s12964-021-00768-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MB49 cells conditioned medium could promote the transformation of BMDM to M2 phenotype. A The expression of Il-10, Cd206, Tgfb and Inos was quantified by qRT-PCR in macrophages stimulated with MB49 cells conditioned medium for 12 and 24 h, respectively. Results are present as the means ± SD of 3 independent experiments. Data were analyzed by one-way ANOVA with multiple comparisons test (*P < 0.05, **P < 0.01, ****P < 0.0001). B BMDM were stimulated with MB49 conditioned medium for 48 h, and the proportion of F4/80⁺CD206⁺ cells was detected by flow cytometry. Representative images of each sample are shown. Data were analyzed by t-test (Mean ± SD, n = 3, ***P < 0.001)