Fig. 3.

Candidate immune-related TEs associated with lower allele-specific expression are not enriched for H3K9me3. A ChIP qPCR analysis for H3K9me3 in the genomic region where FBti0020057, FBti0018883, tdn4, FBti0018877, FBti0019985, and FBti0020137 are inserted. TE (-): H3K9me3 enrichment in the strain that does not contain the TE insertion (grey). Left: H3K9me3 enrichment in the left flanking region of each TE in a strain with the insertion (red). Right: H3K9me3 enrichment in the right flanking region of each TE in a strain with the insertion (red). None of the candidate immune-related TEs tested are enriched for H3K9me3. FBti0020057: p = 0.384 and p = 0.115 for the left and right TE-flanking regions, respectively; FBti0018883: p = 0.473 and p = 0.425, respectively; tdn4: p = 0.408 for the right TE-flanking region; FBti0018877: p = 0.364 and p = 0.632, respectively; FBti0019985: p = 0.041 and p = 0.039, respectively; and FBti0020137: p = 0.880, and p = 0.423, respectively. B FBti0020057 is associated with lower reporter gene expression in both non-infected and infected conditions. Schematic representation of the vector construction with the intergenic region between CG15829 and CG8628 genes with and without FBti0020057 insertion cloned upstream of the reporter gene lacZ. Below, normalized expression levels of the lacZ reporter gene in transgenic female guts with (red) and without (grey) FBti0020057 are shown. On the right side, β-GAL immunostaining (green), and DAPI staining (grey) of guts from transgenic females with and without FBti0020057. Scale bars represent 500 μm