Table 1.
Parameters of the orthogonal tests for phage microencapsulation
| No. | A (%) | B | C (mm) | D (%) | E (rpm) | EE (%) |
|---|---|---|---|---|---|---|
| 1 | 3 | 1:2 | 1.2 | 3 | 0 | 26.710 ± 1.191 |
| 2 | 2 | 1:1 | 0.5 | 3 | 500 | 32.280 ± 0.481 |
| 3 | 1 | 1:2 | 0.9 | 2 | 500 | 29.700 ± 1.018 |
| 4 | 1.5 | 2:1 | 0.5 | 2 | 200 | 41.057 ± 1.592 |
| 5 | 2.5 | 4:1 | 0.7 | 3 | 200 | 30.753 ± 1.005 |
| 6 | 1 | 4:1 | 0.5 | 1 | 0 | 55.423 ± 4.356 |
| 7 | 2 | 4:1 | 0.9 | 4 | 300 | 30.660 ± 0.407 |
| 8 | 1.5 | 1:1 | 0.6 | 4 | 0 | 34.180 ± 1.374 |
| 9 | 1.5 | 1:2 | 0.7 | 1 | 300 | 48.633 ± 1.653 |
| 10a | 3 | 4:1 | 0.6 | 2 | 100 | 77.767 ± 2.636 |
| 11 | 2.5 | 2:1 | 0.9 | 5 | 0 | 27.995 ± 1.270 |
| 12 | 3 | 1:1 | 0.9 | 1 | 200 | 34.028 ± 1.867 |
| 13 | 1.5 | 1:4 | 0.9 | 3 | 100 | 24.120 ± 1.559 |
| 14 | 2.5 | 1:1 | 1.2 | 2 | 300 | 25.707 ± 1.494 |
| 15 | 1.5 | 4:1 | 1.2 | 5 | 500 | 38.097 ± 1.962 |
| 16 | 2 | 2:1 | 1.2 | 1 | 100 | 55.140 ± 1.702 |
| 17 | 2 | 1:2 | 0.6 | 5 | 200 | 30.917 ± 0.820 |
| 18 | 3 | 1:4 | 0.5 | 5 | 300 | 26.576 ± 1.134 |
| 19 | 2.5 | 1:2 | 0.5 | 4 | 100 | 29.537 ± 1.788 |
| 20 | 2.5 | 1:4 | 0.6 | 1 | 500 | 29.930 ± 0.853 |
| 21 | 3 | 2:1 | 0.7 | 4 | 500 | 59.243 ± 2.671 |
| 22 | 1 | 1:1 | 0.7 | 5 | 100 | 25.440 ± 0.421 |
| 23 | 1 | 2:1 | 0.6 | 3 | 300 | 44.530 ± 2.071 |
| 24 | 1 | 1:4 | 1.2 | 4 | 200 | 27.153 ± 1.940 |
| 25 | 2 | 1:4 | 0.7 | 2 | 0 | 29.290 ± 1.095 |
A: concentration of sodium alginate; B: ratio of phage to sodium alginate; C: syringe needle diameter; D: concentration of CaCl2; E: speed of stirring; EE: encapsulation efficiency (mean ± SD, n = 3).
aPreparation parameters with the highest encapsulation efficiency.