Fig. 1.

Successfully established OATP1B1-GFP-HEK293T cell model (A) in which OATP1B1 was highly expressed. Compared to the control GFP-HEK293T cells (B), OATP1B1 expression increased about 214% in the OATP1B1-GFP-HEK293T cells. Both, GFP-HEK293T cells and OATP1B1-GFP-HEK293T cells were cultured in regular DMEM with 10% FBS. OATP1B1expression in the GFP-HEK293 cells and HEK293T-OATP1B1 cells were detected by western blot (C), and showed significant difference between the two cell models. β-actin served as an internal control