Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 9;588(7837):331–336. doi: 10.1038/s41586-020-2969-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s), under exclusive licence to Springer Nature Limited 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — a, Heat maps showing coordinated expression of chr 8p genes mirrored their copy number status in the two large breast cancer datasets, METABRIC and TCGA. The 8p^low cluster is defined by CNA data. The right panel shows distribution of 8p^low cluster in different breast cancer subtypes and its association with disease specific survival. P values calculated using two-sided, log-rank tests. CNA, Copy Number Alteration. Exp, RNA-Seq Expression. b, Hierarchical clustering of primary breast tumours by 8p gene expression in the EMC-MSK dataset. The 8p^low cluster is enriched in tumours that developed brain metastasis, but not lung or bone metastasis. The right panel shows organ-specific metastasis free survival curves stratified by 8p^low status. The 8p^low cluster displays poorer brain metastasis compared to the 8p^WT cluster. Brain metastasis free survival curves stratified by 8p^low status in different subtypes is also presented. P values calculated using two-sided, log-rank tests. c, Hierarchical clustering of breast cancer metastases by 8p gene expression, with the 8p^low cluster being enriched in brain metastases. d, Chr 8p CNA status determined by Targeted Seq in the MSK metastatic breast cancer dataset. Brain metastases are enriched in chr 8p deletion compared to primary tumour, local recurrence and metastases at other sites. The 8p^low cluster predicts poor brain metastasis free survival. P values calculated using two-sided, log-rank tests. LN, lymph node. e, Heat maps showing co-regulated patterns of two independent PI3K-response signatures in METABRIC and TCGA breast cancer datasets. PI3Ksig.1 was generated by overexpression of PIK3CA^mut in breast epithelial cells. PI3Ksig.2 was generated by PI3K inhibitor treatment in the CMap database. The right panel shows distribution of PI3Ksig^high cluster in different breast cancer subtypes and its association with disease specific survival. P values calculated using two-sided, log-rank tests. f, Hierarchical clustering of primary breast tumours by PI3K signatures in the EMC-MSK dataset. The PI3Ksig^high cluster is enriched in tumours that developed brain metastasis. The right panel shows organ-specific metastasis free survival curves stratified by PI3K signatures. The PI3Ksig^high cluster displayed poorer brain metastasis. Brain metastasis free survival curves stratified by PI3K signatures in different subtypes is also presented. P values calculated using two-sided, log-rank tests. g, Hierarchical clustering of breast cancer metastases by PI3K signatures, with the PI3Ksig^high cluster being enriched in brain metastases. h, Heat maps showing significant yet non-complete overlap between 8p^low and PI3Ksig^high clusters in the EMC-MSK dataset. 8p^low and PI3Ksig^high clusters co-capture a subset of patients with the worst brain metastasis prognosis. P values calculated using two-sided, log-rank tests. The lower panel presents a Cox proportional-hazards model of brain metastasis free survival using multi variates – 8p, PI3Ksig, and breast cancer subtype. The 8p^low/PI3Ksig^high cluster is the most associated with brain metastasis. i. 8p^low and PI3Ksig^high clusters co-capture the majority of brain metastasis samples.

Source data