Figure 2.

Measuring DNA BER of oxidative lesions. (A) Representative arrayed comet images. (B) Schematic of the mammalian BER pathway. (C) H₂O₂ dose response curves when exposed to multiple doses of H₂O₂ for 20 min at 4°C for live and lysed (dead or non-viable) TK6 lymphoblasts. (D) H₂O₂ dose response curves for MCL-5 cells without Fpg, with Fpg buffer, and with Fpg enzyme + Fpg buffer. (E) MMS dose response curves for MCL-5 cells without hAAG, with hAAG buffer, and with hAAG enzyme + hAAG buffer. *p < 0.05, Student’s t-test (one-tailed, paired), comparing to WT at each time point (black stars show hAAG compared to WT, grey stars show buffer only compared to WT).