Figure 3.

Measuring DNA damage in the absence of BER proteins. DNA damage in Polβ^−/− (A) and Ogg1^−/− (B) MEFs after treatment with H₂O₂ at 4°C for 20 min. All NT cells were exposed to 1x PBS as control. Data points and error bars represent averages and standard errors of the mean (SEM), respectively, of three independent experiments (n ≥ 3) with at least 100 comets scored for each condition in each experiment. *p < 0.05, Student’s t-test (one-tailed, paired), comparing to WT at each time point.