Figure 4.

Measuring DNA BER of alkylation damage. (A) Damage levels in MEFs for non-treated (NT) cells and immediately after 30 min of treatment with 1 mM MMS at 37°C. (B) Repair kinetics of MEFs after treatment with 1 mM MMS at 37°C for 30 min. NT and 0 min data replotted from (A). Repair was performed in media at 37°C. Data points and error bars represent averages and standard errors of the mean (SEM), respectively, of three independent experiments (n ≥ 3) with at least 100 comets scored for each condition in each experiment. *p < 0.05, Student’s t-test (one-tailed, paired), comparing to WT at each time point.