Table 10.

Scores obtained from eye irritation assessment of different formulations tested on New Zealand white rabbits.

Lesion	Score for each lesion	Control group (non-medicated Carbopol 934 in situ gel formulation)	F1 (optimized NT-Cub dispersed in Carbopol 934 in situ gel base)	F2 (aqueous dispersion of NT-Cub)	F3 (NT powder in Carbopol 934 dispersion)	Commercially available NT aqueous suspension
(A) Conjunctival edema (chemosis)
No swelling	0	0	–	–	–	–
Any swelling	1	–	1	1	1	1
Prominent swelling along with partial lid eversion	2	–	–	–	–	–
Swelling with half-closed lids	3	–	–	–	–	–
Swelling with totally closed lids	4	–	–	–	–	–
(B) Redness in conjunctiva
Absent	0	0	0	–	–	–
Abnormal conjunctival injections	1	–	–	1	–	1
More diffuse and deeper hyperemia, separate vessels cannot be seen easily	2	–	–	–	2	–
Diffuse and dense hyperemia	3	–	–	–	–	–
(C) Secretion
Absent	0	–	–	0	–	–
Any abnormal secretion	1	1	1		1	1
Secretion leading to wet eye lashes closer to lids	2	–	–	–	–	–
Secretion leading to wet lids and whole periorbital area	3	–	–	–	–	–
(D) Corneal opacity
Absent	0	0	0	0	–	–
Scattered or diffuse areas, detail of the iris discernible	1	–	–	–	1	1
Easy discernible, transparent areas, detail of the iris slightly darkened	2	–	–	–	–	–
Opalescent areas, no details of the iris discernible, size of the pupil  barely discernible	3	–	–	–	–	–
Opaque cornea, iris not discernible	4	–	–	–	–	–
(E) Iris involvement
Absent	0	0	0	0
Pronounced deep folds, congestion, deep swelling circumcorneal injection,  the iris still reacts to light	1	–	–	–	1	1
No response, hemorrhage, marked destruction	2	–	–	–	–	–
	Total score	1	2	2	6	5