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. 2021 Sep 12;59(1):1243–1253. doi: 10.1080/13880209.2021.1972121

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Icariin activates the BMP-2/Smad5/Runx2 pathway in the rat model of fractures. (A) RT-qPCR was performed to detect the mRNA expression levels of Runx2, Osterix, BMP-2 and Smad5. GAPDH was used as the control. (B) The expression levels of Runx2, Osterix, BMP-2, p-Smad5 and t-Smad5 proteins were detected by Western blotting. (1) Model group, (2) L-icariin group, (3) M-icariin group and (4) H-icariin group. (C) The relative expression levels of Runx2, Osterix, BMP-2, p-Smad5 and t-Smad5 were calculated by normalizing to the levels of GAPDH. Data are represented as the means ± SD (n = 3). *p< 0.05, **p< 0.01 and ***p< 0.001 vs. the model group. ^#p< 0.05 vs. the corresponding L-icariin group.