Skip to main content
. 2021 Sep 12;59(1):1243–1253. doi: 10.1080/13880209.2021.1972121

Figure 7.

Figure 7.

Icariin activated the BMP-2/Smad5/Runx2 pathway in rBMSCs from young and old rats. (A) RT-qPCR was performed to detect the mRNA expression levels of Runx2, Osterix, BMP-2 and Smad5. GAPDH was used as the control. (B) The expression levels of Runx2, Osterix, BMP-2, p-Smad5 and t-Smad5 proteins were detected by Western blotting. (1) 5% icariin-control group, (2) 5% icariin-containing group, (3) 20% icariin-control group and (4) 20% icariin-containing group. (C) The relative expression levels of Runx2, Osterix, BMP-2, p-Smad5 and t-Smad5 were calculated by normalizing to the levels of GAPDH. Data are represented as the means ± SD (n = 4). *p< 0.05 vs. its icariin-control group. #p< 0.05 vs. the 5% icariin-containing group.