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. Author manuscript; available in PMC: 2021 Sep 14.
Published in final edited form as: Plant J. 2017 Feb 13;89(6):1251–1262. doi: 10.1111/tpj.13446

Figure 3.

Figure 3.

Wheat dwarf virus (WDV) replicon-mediated expression of the CRISPR/Cas9 system for targeted mutagenesis in wheat cells. (a) CRISPR/ Cas reagents expressed from the different architectures of the WDV replicons. (b) PCR/restriction enzyme assay to detect mutations in the ubiquitin gene induced with the sgUbi1 expressed in the different vectors shown in (A); *percentages of NHEJ (% ±SEs) represent the average of two different transfections and have been normalized to the transfection efficiency (40 and 60%, respectively). (c) Sequences of resistant bands obtained by NHEJ of the ubiquitin gene mediated by the pWDV1-CR vector.