Table 2.

Antimicrobial activity of methanol extracts of C. vulgaris stored under four temperature and light regimes for up to 15 months. MIC values (n = 3–6) are shown for both samples. Different letters indicate significant differences between storage time (capital letters, rows) and storage conditions (lowercase letters, columns)

Treatment	Storage time
	T0	T4	T6	T9	T12	T15
	MIC (mg/mL)
Staphylococcus aureus ATCC12600
− 70 °C/dark	4.7; 4.7 B	n.m	3.15; 3.15 C a	3.15; 3.15 C a	0.78; 0.78 D a	12.5; 12.5 A a
10 °C/dark	AB	n.m	3.15; 3.15 B a	0.78; 0.78 B b	0.20; 0.20 B c	12.5; 6.25 A a
25 °C/dark	B	n.m	1.56; 3.9 B a	0.78; 1.56 BC b	0.39; 0.39 D b	12.5; 12.5 A a
25 °C/light	B	n.m	3.9; 3.9 B a	3.15; 3.15 C a	0.78; 0.78 D a	12.5; > 12.5 A a
Neomycin	0.39 µg/mL
Escherichia coli ATCC11775
− 70 °C/dark	> 12.5; > 12.5 A	n.m	12.5; 12.5 A a	> 12.5; > 12.5 A a	12.5; 12.5 A a	> 12.5; > 12.5 A a
10 °C/dark	A	n.m	12.5; 12.5 A a	12.5; 12.5 A a	6.25; 12.5 A a	> 12.5; > 12.5 A a
25 °C/dark	A	n.m	12.5; > 12.5 A a	> 12.5; > 12.5 A a	12.5; 6.25 A a	> 12.5; > 12.5 A a
25 °C/light	A	n.m	12.5; 12.5 A a	12.5; 12.5 A a	6.25; 3.15 B a	> 12.5; > 12.5 A a
Neomycin	1.56 µg/mL

n.m, not measured due to COVID-19 lockdown regulations limiting laboratory access times