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. 2021 Apr 5;2(3):100033. doi: 10.1016/j.xhgg.2021.100033

Figure 3.

Figure 3

Expression of human PTPN4, PTPN4 p.Gly239Arg, and PTPN4 p.Arg808His proteins in rat primary hippocampal neurons

The neurons were transfected with PTPN4-EGFP, PTPN4 p.Gly239Arg-EGFP, PTPN4 p.Arg808His-EGFP, or control EGFP expression vectors and immunostained with antibodies recognizing: GFP (to delineate PTPN4 localization), PSD95 (to show glutamatergic synapses), MAP2 (to visualize dendrites), and DAPI (for nuclear staining). (A–C) An overview of DIV18 rat hippocampal neurons showing PTPN4 and PTPN4 p.Gly239Arg and PTPN4 p.Arg808His protein ubiquitous localization within the cytoplasm and nucleus of neuronal cell body. (A) The PTPN4 protein is also present in axons (designated as white arrow), dendrites, and dendritic spines. (B) The PTPN4 p.Gly239Arg protein is present in dendrites, but it does not localize to dendritic spines. (C) PTPN4 p.Arg808His protein is expressed in both dendrites and dendritic spines. (D) The visualization of neuron transfected with EGFP as a control. Scale bars, 20 μm.