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. 2021 Sep 14;16(9):e0256276. doi: 10.1371/journal.pone.0256276

Fig 3. PACT knockdown induces MBNL1 protein expression.

Fig 3

Western blot analysis of MBNL1 protein levels upon PACT knockdown in fibroblasts from (A) DM1500 CTG HF and (C) DM12000 CTG HF. Corresponding quantification of MBNL1 protein levels in (B) DM1500 CTG HF and (D). Quantification of MBNL1 positive foci in HF DM1500 CTG and DM12000 CTG treated with PACT and scrambled siRNA. (E). The cytoplasmic to nuclear MBNL1 ratio was also quantified (F; details of the MBNL1 signal quantification using the Columbus software are shown in S3 Table). MBNL1 protein level was normalized to HSP90 loading control, averaged across trials and presented as relative fold-change to Non-targeting siRNA (n = 4; unpaired t-test; error bars represent SD). *P < 0.05, **P < 0.01, ***P< 0.001.