Fig 4. PACT knockdown does not impact IR splicing and imparts a partial, non-statistically significant trend towards SERCA1 splicing correction in DM1 cells.
RT-qPCR analysis of (A and C) SERCA1 and (B and D) IR alternative splicing upon PACT knockdown in DM1500 CTG and DM12000 CTG, respectively. The non-targeting siRNA treated sample in one trial was set as the “control” sample and all other samples–meaning all non-targeting siRNA and PACT siRNA treated sample from all other trials–were quantified relative to this “control” (n = 5; unpaired t-test; error bars represent SD).