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Small molecule PKR inhibitor C16 was used to treat (A) DM1-500500CTG HF or (B) DM12000cTG HF at doses ranging from 0.1nM-10,000nM for 8hrs up to 168hrs (7 days) following which (CUG)n foci were detected by RNA FISH and foci number and area quantified using Columbus software (n = 3; two-way ANOVA; error bars represent SD). *P < 0.05, **P < 0.01, ***P< 0.001.
