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. 2021 Sep 14;16(9):e0256870. doi: 10.1371/journal.pone.0256870

Fig 3. The effect of morphine and LPS on microglial activation by immunocytochemical analysis.

Fig 3

(a) BV-2 cells were treated with PBS, DMSO, LPS (1 μg/mL), morphine (200 μM), LPS (1 μg/mL) + morphine (200 μM), morphine (200 μM) + AG1478 (10 μM), and LPS (1 μg/mL) + morphine (200 μM) + AG1478 (10 μM) for 6 h. Cells were stained for the activation marker CD11b. Scale bar: 20 μm. (b) The mean optical densities of six randomly selected fields of cells from each group were analyzed by ImageJ software for quantitative analysis. Data are expressed as the means ± SEMs. All data were analyzed using ANOVA. * p < 0.05 and ** p < 0.01 compared to the control groups. # p < 0.05 and ## p < 0.01 morphine vs. morphine + AG1478. & p < 0.05 and && p < 0.01 LPS + morphine vs. LPS + morphine + AG1478.