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. 2021 Aug 31;12:739025. doi: 10.3389/fimmu.2021.739025

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Copyright © 2021 Geerling, Stone, Steffen, Hassert, Brien and Pinto

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The obese state in females promotes early WNV entry into peripheral organs. (A–D) Infectious viral titers in female mouse organs. Mice were infected with 100 FFU of WNV via subcutaneous foot pad injection. At 3 (wt n=6 and ob n=6), 8 (wt n=5 and ob n=5) or 15 (wt n=4 and ob n=4) days post infection, liver (A), spleen (B), fat (C) and kidney (D) were harvested, frozen and homogenized. Levels of infectious virus were measured via focus forming assay and reported as FFU/ml of organ homogenate. Asterisks indicate statistically significant values (*p < 0.05) as determined by Mann-Whitney test. ns, not significant.