(A) Concentration-response curves to ACh in the presence or absence of COX-1 inhibitor, SC560 (no PVATmedia added); (B) Concentration-response curves to ACh in the presence or absence of SC560 (PVATmedia added); (C) Area under the curve for all concentration-response curves in (A) and (B); (D) Concentration-response curves to ACh in the presence or absence of COX-2 inhibitor, NS398 (no PVATmedia added); (E) Concentration-response curves to ACh in the presence or absence of NS398 (PVATmedia added); (F) Area under the curve for all concentration-response curves in (D) and (E). Values are mean ± SEM. One-way ANOVA followed by Tukey’s post hoc test. ACh, acetylcholine; COX, cyclooxygenase; PVATmedia, perivascular adipose tissue conditioned media.