Table 1. Primers used for ChIP.
Gene | Accession # | Primers | Sequence | Amplicon (bp) |
---|---|---|---|---|
Kiss1 | NM_181692.1 | rKiss1p CHIP-F | TCGGGCAGCCAGATAGAGGAAGC | 91 |
rKiss1p CHIP-R | TTGAGGGCCGAGGGAGAAGAG | |||
Kiss1 | NM_181692.1 | rKiss1e CHIP-F | CCAGCCCGGGGGATGGGGTGTAA | 192 |
rKiss1e CHIP-R | AGGGGCCAGCGCAGCAGCACAAT | |||
Tac3 | NM_019162.2 | rTAC2p CHIP-F | ACGTGCGTGTCTGGGTATGTGA | 123 |
rTAC2p CHIP-R | GGAGGGTTTGGGGGAGTCG | |||
rPCSK1p CHIP-R | CCTTCGAGACAGCATTCACA |
F, forward; R, reverse.