Table 2. Primers used for real-time RT-PCR.
| Gene | Accession # | Primers | Sequence |
|---|---|---|---|
| Hprt | NM_013556.2 | Hprt-F | CCTGCTGGATTACATTAAAGCGCTG |
| Hprt-R | GTCAAGGGCATATCCAACAACAAAC | ||
| Kiss1 | AF472576.1 | Kiss1-F | GCTGCTGCTTCTCCTCTGTG |
| Kiss1-R | TCTGCATACCGCGATTCCTT | ||
| Tac1 | NM_009311.2 | Tac1-F | ATGAAAATCCTCGTGGCCGT |
| Tac1-R | GTTCTGCATCGCGCTTCTTT | ||
| Pdyn | NM_018863.4 | Pdyn-F | ACAGGGGGAGACTCTCATCT |
| Pdyn-R | GGGGATGAATGACCTGCTTACT | ||
| Tacr3 | NM_021382.6 | Tacr3-F | GCCATTGCAGTGGACAGGTAT |
| Tacr3-R | ACGGCCTGGCATGACTTTTA | ||
| Nhlh2 | NM_178777.3 | Nhlh2-F | ACCAGAAGAGCCAAGAAGCCA |
| Nhlh2-R | GCGGGTGTATGGTTGTTCACTTAG |
F, forward; R, reverse.