Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | actin-GAL4 | Bloomington | 25374 | y[1] w[*]; P{Act5C-GAL4-w}E1/CyO |
| Genetic reagent (Drosophila melanogaster) | tubulin-GAL4 | Bloomington | 5138 | y[1] w[*]; P{w[+mC]=tubP-GAL4}LL7/TM3, Sb[1] Ser[1] |
| Genetic reagent (Drosophila melanogaster) | elav-GAL4 | Bloomington | 77894 | y[1] w[*] P{w[+mC]=elav(FRT.ewg)GAL4.eFeG}1 |
| Genetic reagent (Drosophila melanogaster) | nrv-GAL4 | Bloomington | 6799 | w[*]; P{w[+mC]=nrv2-GAL4.S}8 |
| Genetic reagent (Drosophila melanogaster) | D42-GAL4 | Bloomington | 8816 | w[*]; P{w[+mW.hs]=GawB}D42 |
| Genetic reagent (Drosophila melanogaster) | n-syb-GAL4 | Bloomington | 51635 | y[1] w[*]; P{w[+m*]=nSyb-GAL4.S}3 |
| Genetic reagent (Drosophila melanogaster) | repo-GAL4 | Bloomington | 7415 | w[1118]; P{w[+m*]=GAL4} repo/TM3, Sb[1] |
| Genetic reagent (Drosophila melanogaster) | 69B-GAL4 | Bloomington | 1744 | w[*]; P{w[+mW.hs]=GawB}69B |
| Genetic reagent (Drosophila melanogaster) | Mhc-GAL4 | Bloomington | 38464 | ; w[*]; P{w[+mC]=Mhc-RFP.F3-580} |
| Genetic reagent (Drosophila melanogaster) | Mef2-GAL4 | Bloomington | 26882 | w[*]; Kr[If-1]/CyO, P{w[+mC]=GAL4-Mef2.R}2, P{w[+mC]=UAS-mCD8.mRFP} |
| Genetic reagent (Drosophila melanogaster) | c179-GAL4 | Bloomington | 6450 | w[*]; P{w[+mW.hs]=GawB}c179 |
| Genetic reagent (Drosophila melanogaster) | how24B-GAL4 | Bloomington | 1767 | w[*]; P{w[+mW.hs]=GawB}how[24B] |
| Genetic reagent (Drosophila melanogaster) | GMR-GAL4 | Bloomington | 9146 | w[1118]; P{GMR-GAL4.w[-]}2/CyO |
| Genetic reagent (Drosophila melanogaster) | nub-GAL4 | Bloomington | 86108 | w[*]; P{w[nub.PK]=nub-GAL4.K}2 |
| Genetic reagent (Drosophila melanogaster) | 5053 GAL4 | Bloomington | 2702 | w[*]; P{w[+mW.hs]=GawB}tey[5053A]/TM6B, Tb[+] |
| Genetic reagent (Drosophila melanogaster) | srmd710-GAL4 | Bloomington | 26663 | w[*]; P{w[+mW.hs]=GawB}sr[md710]/TM6B, Tb[1] |
| Genetic reagent (Drosophila melanogaster) | UAS dCNBPRNAi | VDRC | GD16283 | CNBP long hairpin on chromosome X |
| Genetic reagent (Drosophila melanogaster) | UAS dCNBPRNAi | VDRC | GD16284 | CNBP long hairpin on chromosome 2 |
| Genetic reagent (Drosophila melanogaster) | 2XUAS dCNBPRNAi | Antonucci et al., 2014 | GD16283+ GD16284 | - |
| Genetic reagent (Drosophila melanogaster) | dCNBP k | Kyoto | 203535 | y[1] w[67c23]; P{w[+mC]=GSV6}GS11716 / SM1 |
| Genetic reagent (Drosophila melanogaster) | UAS dCNBP-HA RNAi resistant | This study | – | Injection stock #BL 8622 |
| Genetic reagent (Drosophila melanogaster) | UAS hCNBP-FLAG | This study | – | Injection stock #BL 8622 |
| Genetic reagent (Drosophila melanogaster) | UAS Odc1RNAi | VDRC | GD30039 | Odc1 long hairpin on chromosome 3 |
| Genetic reagent (Drosophila melanogaster) | UAS Odc1RNAi | VDRC | GD30038 | Odc1 long hairpin on chromosome 2 |
| Genetic reagent (Drosophila melanogaster) |
UAS Odc2RNAi | VDRC | KK104597 | Odc2 long hairpin on chromosome 2 |
| Genetic reagent (Drosophila melanogaster) | UAS Odc1+Odc2RNAi | This study | GD30038+ KK104597 | Long hairpin for Odc2 on chromosome 2 and for Odc1 on chromosome 3 |
| Genetic reagent (Drosophila melanogaster) | dOdc1 mutant | Bloomington | 56103 | y[1] w[*]; Mi{y[+mDint2]=MIC}Odc1[MI10996] |
| Genetic reagent (Drosophila melanogaster) | UAS dOdc1 | Gupta et al., 2013 | - | - |
| Antibody | anti-CNBP (goat) |
Abcam | ab48027, RRID:AB870003 | WB 1:1000 |
| Antibody | anti-ODC (rabbit) |
ENZO | BML-PW8880-0100 RRID:AB_2156495 |
WB 1:500 |
| Antibody | anti-Actin goat | Santa Cruz | sc-1616, RRID:AB630836 | WB 1:3000 |
| Antibody | anti-GFP (mouse) |
Santa Cruz | sc-9996, RRID:AB_627695 | WB 1:3000 |
| Antibody | anti-Vinculin (mouse) |
Santa Cruz | sc-73614, RRID:AB_1131294 | WB 1:3000 |
| Antibody | anti-FLAG-HRP | Sigma | A8592, RRID:AB_439702 | WB 1:500 |
| Antibody | anti-CNBP (mouse) |
Agrobio (this study) |
– | WB 1:1000 |
| Antibody | anti-HA-HRP (mouse) |
Santa Cruz | sc-7392, RRID:AB_627809 | WB 1:2000 |
| Antibody | anti-vibrator (rabbit) | Giansanti et al., 2006 | - | WB 1:3000 |
| Sequence-based reagent |
T7 CNBP FW
Drosophila melanogaster |
This study | dsRNA primer | TAATACGACTCACTATAGGGAG GTCCGGGCGGCGTTGG |
| Sequence-based reagent |
T7 CNBP RV
Drosophila melanogaster |
This study | dsRNA primer | TAATACGACTCACTATAGGGAG ATGTGTCCGGTGCGG |
| Sequence-based reagent |
dOdc1 Fw
Drosophila melanogaster |
This study | PCR primer | TGGCAGCGATGACGTAAAGTT |
| Sequence-based reagent |
dOdc1 Rv
Drosophila melanogaster |
This study | PCR primer | TGGTTCGGCGATTATGTGAA |
| Sequence-based reagent |
GAPDH Fw
Drosophila melanogaster |
This study | PCR primer | CCTGGCCAAGGTCATCAATG |
| Sequence-based reagent | GAPDH RvDrosophila melanogaster | This study | PCR primer | ATGACCTTGCCCACAGCCTT |
| Sequence-based reagent | dOdc1-IRES FW | This study | PCR primer | TAAGAATTCCTCGGAAAGATCTCAAC |
| Sequence-based reagent | dOdc1-IRES RW | This study | PCR primer | TTAGAATTCACAAGTCGT TGACTGATAAC |
| Chemical compound, drug | DFMO | Sigma | #D193 | |
| Commercial assay or kit | RevertAid H Minus First Strand cDNA Synthesis kit | Thermo Fisher Scientific | K1632 | |
| Chemical compound, drug | Putrescine | Sigma | #51799 | |
| Chemical compound, drug | Spermidine | Sigma | #S2626 | |
| Plasmids | plko SCR, plkoSh_cnbp | D'Amico et al., 2015 | ||
| Plasmids | GFP | Coni et al., 2020 | ||
| Plasmids | hODC-LUC | D'Amico et al., 2015 | ||
| Plasmids | dOdc1-LUC | This study | ||
| Plasmids | HA-dCNBP | Antonucci et al., 2014 | ||
| Plasmids | FLAG-hCNBP | D'Amico et al., 2015 | ||
| Cell line | S2 | DGRC | Cat# 181, RRID:CVCL_Z992 | |
| Cell line | HEK-293T | ATCC | CRL-3216, RRID:CVCL_0063 |