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. 2021 Aug 19;10:e70272. doi: 10.7554/eLife.70272

Figure 7. C- and N-terminal tagging of TbFUT1 result in mitochondrial and Golgi apparatus localization, respectively.

Figure 7.

(A) Bloodstream form (BSF) cKO parasites expressing tet-inducible C-terminally tagged TbFUT1-MYC3 were imaged under permissive (+Tet) and non-permissive (-Tet) conditions by differential interference contrast (DIC) and fluorescence microscopy after staining with anti-MYC, MitoTracker, and DAPI. Comparable patterns were observed for anti-MYC and MitoTracker, suggesting that TbFUT1-MYC3 localizes to the mitochondrion. (B, C) IFA of BSF cKO parasites expressing a tet-inducible N-terminally tagged HA3-TbFUT1-MYC3 (B) or HA3-TbFUT1 (C) after labelling with anti-HA, anti-GRASP, and DAPI suggests a Golgi apparatus location for both HA3-TbFUT1-MYC3 and HA3-TbFUT1. The absence of anti-MYC (A) or anti-HA (B, C) staining under non-permissive conditions confirms the specificity of the labelling for the respective TbFUT1 fusion proteins. White boxes: areas magnified 1.5× in the rows below. Scale bars: 3 μm.