| Reagent type (species) or resource |
Designation |
Source or reference |
Identifiers |
Additional information |
| Gene (Trypanosoma brucei) |
TbFUT1
|
Genedb.org
|
Tb927.9.3600 |
|
| Strain, strain background (Trypanosoma brucei) |
Strain 427, clone 29.13 (PCF WT) |
Wirtz et al., 1999
|
|
Procyclic form |
| Strain, strain background (Trypanosoma brucei) |
Strain 427, variant MITaT 1.2 (BSF WT) |
Wirtz et al., 1999
|
|
Bloodstream form |
| Strain, strain background(Leishmania major) |
L. major Friedlin V1 |
Guo et al., 2017
|
MHOM/IL/80/Friedlin |
|
| Strain, strain background (Escherichia coli) |
BL21 (DE3) |
Agilent |
|
|
| Strain, strain background (Escherichia coli) |
BL21 Gold(DE3) |
Agilent |
|
|
| Recombinant DNA reagent |
pLEW100 |
Prof. George Cross (Rockefeller University) |
Addgene Plasmid #24011 |
(Plasmid) |
| Recombinant DNA reagent |
pMOTag4YH-PAC |
Prof. Thomas Seebeck (University of Bern); Oberholzer et al., 2006
|
|
(Plasmid) |
| Recombinant DNA reagent |
pIR1NEO |
Guo et al., 2021
|
|
(Plasmid) |
| Recombinant DNA reagent |
pGEX6P1-GST-PP |
Prof. Daan Van Aalten (University of Dundee) |
|
(Plasmid) |
| Recombinant DNA reagent |
pET15b |
Novagen |
|
(Plasmid) |
| Chemical compound, drug |
GDP[3H]Fuc |
American Radiochemicals |
|
|
| Chemical compound, drug |
Galβ1,3GlcNAcβ-OMe (LNB-O-Me) |
Toronto Research Chemicals |
|
|
| Peptide, recombinant protein |
X. manihotis α–1,2-fucosidase |
New England Biolabs |
|
|
| Commercial assay or kit |
GDP-Glo Kit |
Promega |
|
|
| Other |
MitoTracker Red CMX Ros |
Thermo Fisher |
|
|
| Antibody |
(Rabbit polyclonal) anti-TbFUT1 IgG |
This study: antibody generated as described in Materialsandmethods |
|
(1:1000) |
| Antibody |
(Rabbit polyclonal) anti-GRASP IgG |
Prof. Graham Warren (University College London)/Prof. Chris de Graffenreid (Brown University) |
|
(1:1000) |
| Antibody |
(Rabbit polyclonal) anti-ATPase IgG |
Prof. David Horn (University of Dundee) |
|
(1:1000) |
| Antibody |
(Mouse polyclonal) anti-FAZ IgM |
Prof. Keith Gull (University of Oxford) |
|
(1:2) |
| Antibody |
(Mouse polyclonal) anti-PFR IgG |
Prof. Keith Gull (University of Oxford) |
|
(1:10) |
