Table 1. Acceptor substrates and semi-quantitative fucosyltransferase activities.
| TbFUT1 activity | Lane of Figure 1 | Abbreviations* | Name | Structure |
|---|---|---|---|---|
| +++ | 2, 6 | LNB | Lacto-N-biose | Galβ1,3GlcNAc |
| +++ | 21 | LNB-OMe | Lacto-N-biose-O-methyl | Galβ1,3GlcNAcβ-OMe |
| ++ | 11 | LNT | Lacto-N-tetraose | Galβ1,3GlcNAcβ1,3Galβ1,4Glc |
| ++ | 14 | LNH | Lacto-N-hexaose | Galβ1,3GlcNAcβ1,3(Galβ1,4GlacNAcβ1,6)Galβ1,4Glc |
| ++ | 1,5 | Lac | Lactose | Galβ1,4Glc |
| + | 13 | iLNO | Iso-lacto-N-octaose | Galβ1,3GlcNAcβ1,3(Galβ1,3GlcNAcβ1,3Galβ1,4GlcNAcβ1,6)Galβ1,4Glc |
| + | 3,7 | LacNAc | N-acetyllactosamine | Galβ1,4GlcNAcN |
| + | 10 | LNnH | Lacto-N-neohexaose | Galβ1,4GlcNAcβ1,3(Galβ1,4GlcNAcβ1,6)Galβ1,4Glc |
| + | 12 | LNnT | Lacto-N-neotetraose | Galβ1,4GlcNAcβ1,3Galβ1,4Glc |
| + | 16 | 3′-FL | 3′-Fucosyllactose | Galβ1,4(Fucα1,3)Glc |
| – | 9 | β-Gal | β-Galactose | β-Gal |
| – | 15 | GNG | β1,6-Galactosyl-N-acetyl-glucosamine | Galβ1,6GlcNAc |
| – | 17 | 1,6 GB | β1,6-Galactobiose | Galβ1,6Gal |
| – | 18 | 1,4 GB | Galabiose | Galα1,4Gal |
| – | 19 | LB2TS | Linear B2 trisaccharide | Galα1,3Galβ1,4GlcNAc |
| – | 20 | β-Gal-OMe | β-Galactose-O-methyl | β-Gal-OMe |
| – | 22 | 2’-FL | 2’-Fucosyllactose | Fucα1,2Galβ1,4Glc |
*
The relative efficiencies of the acceptors as TbFUT1 substrates (+++, ++, +, and –) are based on visual inspection of the intensities of the products bands in Figure 2.