Table 2. Partially methylated alditol acetates (PMAAs) derivatives identified by GC-MS methylation linkage analysis of the purified TbFUT1 reaction product.
PMAA derivative | RT (min) | Origin |
---|---|---|
4,6-di-O-methyl-1,3,5-tri-O-acetyl-(1–2H)- 2-N-methylacetamidoglucosaminitol | 24.6 | 3-O-substituted GlcNAc |
2,3,4,6-tetra-O-methyl-1,5-di-O-acetyl-(1–2H)-galactitol | 16.7 | Non-reducing terminal Gal |
3,4,6-tri-O-methyl-1,2,5-tri-O-acetyl-(1–2H)-galactitol | 18.6 | 2-O-substituted Gal |
2,3,4-tri-O-methyl-1,5-di-O-acetyl-(1–2H)-fucitol | 14.1 | Non-reducing terminal Fuc |
3,4-di-O-methyl-1,2,5-tri-O-acetyl-(1–2H)-fucitol | 15.9 | 2-O-substituted Fuc |
RT: retention time.