Table 3. 1H-NMR and 1H-ROESY chemical shift assignments for the purified TbFUT1 reaction product.
Residue | H1 | H2 | H3 | H4 | H5 | H6/6' | NAc |
---|---|---|---|---|---|---|---|
αFuc | 5.05 (J = 4 Hz) | 3.57 | 3.67 | 3.63 | 4.2 | 1.1 | |
βGal | 4.5 | 3.45 | 3.55 | 3.89 | ND | ND | |
βGlcNAc | ND | 3.63 | ND | 3.4 | ND | 3.78/3.89 | 2.1 |
J: coupling constant; ND: chemical shift could not be clearly assigned.