Figure 1. Sensory nerve denervation induces NPY expression.

(A) Representative images of GFP+ neurons in the hypothalamus after multisynaptic tracer HSV-1 H19-G4 injected in the femur marrow for 5 days (dpi, days post injection). (B) Enzyme-linked immunosorbent assay (ELISA) analysis of NPY level in serum in 3-month-old Ntrk1^fl/fl and Avil^Cre:Ntrk1^fl/fl mice. (C) Representative images of immunofluorescence staining and quantitative analysis of NPY (green) in the ARC of hypothalamus of 3-month-old Ntrk1^fl/fl and Avil^Cre:Ntrk1^fl/fl mice. DAPI stains nuclei blue. Scale bars = 50 µm. (D) Quantitative analysis of food intake for 2- and 4-month-old Ntrk1^fl/fl and Avil^Cre:Ntrk1^fl/fl mice. (E) Quantitative analysis of body weight for male and female Ntrk1^fl/fl and Avil^Cre:Ntrk1^fl/fl mice at 1, 3, 6, and 12 months old. (F) Quantitative analysis of the weight of the gonadal and inguinal fat pads isolated from 3-month-old Ntrk1^fl/fl and Avil^Cre:Ntrk1^fl/fl mice. qNMR analysis of (G) fat weight, fat mass, and (H) lean mass of 1- and 3-month-old Ntrk1^fl/fl and Avil^Cre:Ntrk1^fl/fl mice. (I) ELISA analysis of serum NPY level of 3-month-old Avil^Cre:Rosa26 ^lsl-DTR mice injected with vehicle or 1 ug/kg/d of DTX for 5 and 7 days. (J) Representative images of immunofluorescence staining and quantitative analysis of NPY (green) in the ARC of hypothalamus of 3-month-old Avil^Cre:Rosa26 ^lsl-DTR mice injected with vehicle or 1 µg/kg/d DTX for 7 days. Scale bars = 50 µm. (K) Quantitative analysis of food intake for 3-month-old Avil^Cre:Rosa26 ^lsl-DTR mice injected with vehicle or DTX for 1 month. (L) Quantitative analysis of the weight of the gonadal and inguinal fat pads isolated from 3-month-old Avil^Cre:Rosa26 ^lsl-DTR mice injected with vehicle or DTX for 1 month. qNMR analysis of (M) fat weight, fat mass, and (N) lean mass of 3-month-old Avil^Cre:Rosa26 ^lsl-DTR mice injected with vehicle or DTX for 1 month. N ≥ six per group. *p < 0.05, and N.S. means not significant. Statistical significance was determined by Student’s t-test.

Figure 1—figure supplement 1. Knockout efficiency of Avil^Cre:Ntrk1^fl/fl mice.

(A) Representative images of immunostaining for NeuN (Green) and TrkA (Red) and co-staining for NeuN (Green) and TrkA (Red) for the Dorsal Root Ganglion (DRG) of 3-month-old male Ntrk1^fl/fl and Avil^Cre:Ntrk1^fl/fl mice, scale bar:100 µm. (B) Representative μCT images of femurs from 3-month-old male Ptger4^fl/fl and Avil^Cre:Ntrk1^fl/fl mice injected with vehicle or 10 mg/kg/day SW033291 for one month. N≥six per group, *p<0.05 (Student t-test).