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. 2021 Jul 23;7(8):e734. doi: 10.1097/TXD.0000000000001175

FIGURE 4.

FIGURE 4.

Suppression of NETosis by human CD177. PEC and PEC/hCD177 were cultured in 24 well gelatin-coated plates at a concentration of 1.6 × 105 cells/well. After 24 h culturing, PEC and PEC transfectants were cocultured with 3 × 105 neutrophils for 2 h in the presence of 50 nmol/L PMA. The cells were procured and stained with PE-labeled anti-CD66b and 50 nmol/L SYTOX green. Neutrophils were gated in CD66b positive, as shown in the black box. Representative data are demonstrated in (A, B), and a significantly decreased %NETosis was detected in the neutrophils that were cocultured with PEC/hCD177 (C). A significant decrease of SYTOX Green positive dead cells was detected in CD66b positive neutrophils that were cocultured with PEC/hCD177 compared with neutrophils with PEC. The bars indicate the SEM, *P < 0.05, vs PEC, n = 5. PE, phycoerythrin; PEC, porcine endothelial cells; PMA, phorbol 12-myristate 13-acetate.