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. 2021 Aug 16;134(17):2048–2053. doi: 10.1097/CM9.0000000000001687

Figure 2.

Figure 2

Amplification plots of SARS-CoV-2 RNA serial ten-fold dilutions. Dilutions ranged between 5 and 5 × 106 SARS-CoV-2 RNA copies/reaction for the ORF1ab, N, N9, and RdRp assays. Linear correlation coefficients (R2) and efficiencies for each rRT-PCR assay were determined (3 tests/dilution). N: Nucleocapsid; ORF: open reading frames; RdRp: RNA-dependent RNA polymerase; rRT-PCR: Real-time reverse transcription-polymerase chain reaction; SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2.