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. 2021 Sep 3;2021:5594659. doi: 10.1155/2021/5594659

Figure 4.

Figure 4

Potential role of ENST00000585297 in pemphigus. (a) RNA FISH assay of ENST00000585297 in pemphigus. (b) qRT-PCR analysis of nuclear and cytoplasmic RNAs showed that ENST00000585297 was preferentially localized within the cytoplasm. (c) The ceRNA network. The blue circle node indicates mRNA; the yellow circle node indicates miRNA; the red diamond indicates lncRNA. (d) GO analysis of predicted mRNA. (e) Pathway analysis of predicted mRNA. (f) ENST00000585297-siRNA efficiently interferes with ENST00000585297 expression in PBMCs (n = 3). (g) The relative expression of miR-584-3p between the NC-siRNA and ENST00000585297-siRNA groups (n = 5), measured by quantitative reverse-transcription PCR. (∗∗P < 0.001 and ∗∗∗∗P < 0.0001).