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. Author manuscript; available in PMC: 2021 Sep 14.
Published in final edited form as: J Allergy Clin Immunol. 2020 Feb;145(2):550–562. doi: 10.1016/j.jaci.2019.10.031

FIG 5.

FIG 5.

miR-1 targets controlling eosinophil trafficking. A, HUVECs were transduced with V-miR-1 or V-ctrl vectors. Cell lysates were immunoprecipitated with anti–Argonaute2 (Ago2) antibody, and levels of mRNAs bound to Ago2 were measured by using quantitative RT-PCR in the whole lysate (input) and Ago2 immuno-precipitates (AgoIP). RISC recruitment was calculated as mRNA levels in AgoIP/input (2−ΔΔCt, n = 4 per group). *P < .05. B, Expression levels of the target gene mRNAs in V-miR-1– and V-ctrl-transduced HUVECs were normalized to their means in the control (V-ctrl) group and presented as 2−ΔΔCt (n = 4). *P < .05. C, Endothelial cells were isolated from OVA-challenged mice by using magnetic immune sorting, as described in Fig 1, E, and mRNA expression levels were measured, normalized, and presented as described in Fig 5, B (CCL26, SELP, and TSLP: n = 7WT and 8 miR-1 TG; MPL: n = 10 per group; from 2 experiments). *P < .05 and **P = .005331. CSF2, Colony-stimulating factor 2; DSG1, desmoglein 1; ITGA4, integrin subunit α4; POSTN, periostin. Error bars represent SEMs. Data were assessed by using the Student unpaired t test.