FIG. 3.

Expression of Hsk1 and Him1 transcripts and proteins during the cell cycle. (A) cdc25 cells were arrested at the restrictive temperature for 2.5 h and then released into growth at the permissive condition (25°C). Cells were harvested every 20 min, and mitotic and septation index were measured at each time point. (B) RNA was prepared from each culture, and Northern blot analysis was conducted with the probe indicated in the figure. (C) Whole-cell extracts were prepared from cells at each time point and analyzed by Western blotting to detect Him1, Hsk1, and α-tubulin proteins.