FIG. 5.

Him1 protein is hyperphosphorylated in response to cell cycle arrest at S phase. (A) Whole-cell extracts were prepared from the wild type and various cdc mutants grown at a permissive temperature or arrested by incubation at a nonpermissive temperature for 3 h and were run on 8% SDS-PAGE, followed by Western blotting to detect the proteins indicated. (B) Concentrated extracts were prepared from the wild-type fission yeast cells at various times after the addition of HU (10 mM), and analyzed by Western blotting by using an antibody raised against the protein indicated to the left of each panel.