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. 2021 Sep 10;14:4791–4804. doi: 10.2147/OTT.S317122

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© 2021 Chen et al.

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PHA-665752 induces G0/G1 phase arrest and promotes cell apoptosis in OS cells. (A) Cell cycle assay of 143B and U2OS with control or PHA-665752. (n=3) *p<0.05, **p<0.01, ***p<0.001. (B) The expression of Cyclin D1 and CDK4 in 143B and U2OS was detected by Western blot analysis. Cells were treated with different concentration of PHA-665752. (C) TUNEL staining assay of 143B and U2OS with control or PHA-665752. Scale bars, 100μm and 200μm. (n=3) *p<0.05, **p<0.01.