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. 2021 Jun 3;413(24):6159–6170. doi: 10.1007/s00216-021-03375-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Effect of incubation time on the homogeneous assay performance using microcystin-LR (MC-LR) as standard. In BSA-coated microtiter wells, MC-LR (final concentration in 80-μL reaction well: 0.05 to 250 μg/L, plotted in logarithmic scale in X axis) was used to generate TR-FRET signal of the sensitized emission of AF680 at 730 nm (plotted in logarithmic scale in Y axis) at different incubation time points (2–60 min). Standard deviations of duplicate measurements are shown as error bar. Error bars are not visible when interfering with symbols