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. 2021 Sep 15;148(6):1481–1492.e2. doi: 10.1016/j.jaci.2021.09.008

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© 2021 The Authors

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig 2 — SARS-CoV-2–specific ELISPOT assay. PBMCs were isolated from convalescent patients at 1 year postinfection and stimulated with medium, SARS-CoV-2 peptide pool, or PHA for 18 to 24 hours. Then, the number of SARS-CoV-2–specific T cells was measured by IFN-γ ELISPOT assay. A, Representative graphs showing the numbers of SARS-CoV-2–specific T cells in HCs and convalescent patients with COVID-19 with moderate or severe disease. B, The numbers of SARS-CoV-2–specific T cells (SFCs/2.5 × 10⁵ PBMCs) in different groups were expressed as median with IQR. C, Correlation between SARS-CoV-2–specific T-cell numbers and nAb titers. D, Correlation between SARS-CoV-2–specific T-cell numbers and anti-RBD antibody levels. HC, Healthy control; IQR, interquartile range; SFC, spot-forming cell. ∗∗P < .01.