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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Transl Res. 2021 May 15;237:1–15. doi: 10.1016/j.trsl.2021.05.002

Figure 2. Proteomic analysis identifies A3B-pVHL interaction.

Figure 2.

8 million 293T cells were cultured in 10 cm plates, and transfected on the following day with 10 μg of an HA-tagged A3B expression plasmid, or an empty control vector, in the presence of Epoxomicin. Lysates were subsequently incubated with HA- or IgG- conjugated agarose beads in order to affinity-purify A3B and identify non-specific protein-bead interactions, respectively. Proteins were denatured and separated on SDS-PAGE gels. Mass spectrometry analysis identified 39 interacting proteins, as shown, with shading indicating those that were related to ubiquitination and/or the proteasome.