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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Transl Res. 2021 May 15;237:1–15. doi: 10.1016/j.trsl.2021.05.002

Figure 3. Down-regulation of pVHL increases endogenous A3B protein levels.

Figure 3.

MCF7 cells were cultured in 10 cm plates and, one day later, were treated with non-targeting siRNA (siNT) and siRNAs targeting VHL (siVHL 1, 2) in order to knockdown endogenous pVHL. (A, B) Endogenous A3B and pVHL protein levels assessed by immunoblot (n=3) (A) and bands quantified and normalized to the siNT control (B). (C) RT-qPCR analysis of samples from (A) quantifying A3B and VHL mRNA, normalized to TBP. For all panels, values are means ± SD error bars.