Skip to main content
. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Transl Res. 2021 May 15;237:1–15. doi: 10.1016/j.trsl.2021.05.002

Figure 4. Ectopic expression of pVHL decrease levels of A3 proteins.

Figure 4.

800,000 293T cells were seeded on 6-well plates one day prior to co-transfection with plasmids expressing the indicated tagged A3 proteins (500 ng) along with N-terminal Flag-tagged pVHL (1000 ng). Cells were collected 24 hrs post-transfection, lysed, and then separated on 4-12% Bis-Tris gradient gels. A3 and pVHL signals were normalized to the Lamin C band signal. (A) Representative immunoblots of ectopically expressed A3 proteins with co-transfected empty control and Flag-VHL plasmids. (B) Multiple replicate samples of A3B and A3G that were run simultaneously from the experiment illustrated in (A) were quantified and normalized to the Lamin C band signal to yield a degradation ratio of normalized A3B to A3G signals (n=11), values are means ± SD error bars. A Wilcoxon matched-pairs signed rank test determined P value of 0.0322 for comparison of degradation rations of A3B to A3G.